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reverse transcribed intocdnawith thehigh capacity cdnareverse transcription kit  (TaKaRa)


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    Structured Review

    TaKaRa reverse transcribed intocdnawith thehigh capacity cdnareverse transcription kit
    Reverse Transcribed Intocdnawith Thehigh Capacity Cdnareverse Transcription Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15023 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+reverse-transcribed+reagent+kit/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/pm38065409-82-2-9
    Average 99 stars, based on 15023 article reviews
    reverse transcribed intocdnawith thehigh capacity cdnareverse transcription kit - by Bioz Stars, 2026-10
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    Extraction:

    Article Title: Engineering a compact CgCas12n platform for efficient genome and base editing in mammalian cells.
    Article Snippet: .. mRNA extraction, real-time quantitative PCR, and RNA-seq Total RNA was extracted from ~1 × 106 sorted cells using TRIzol reagent (Vazyme, #R401-01) and reverse-transcribed using the PrimeScript RT reagent Kit (TaKaRa, #RR037A). .. Quantitative PCR (qPCR) was performed using SYBR Green Mix (ABclonal, #RK21203) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    Real-time Polymerase Chain Reaction:

    Article Title: Engineering a compact CgCas12n platform for efficient genome and base editing in mammalian cells.
    Article Snippet: .. mRNA extraction, real-time quantitative PCR, and RNA-seq Total RNA was extracted from ~1 × 106 sorted cells using TRIzol reagent (Vazyme, #R401-01) and reverse-transcribed using the PrimeScript RT reagent Kit (TaKaRa, #RR037A). .. Quantitative PCR (qPCR) was performed using SYBR Green Mix (ABclonal, #RK21203) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    RNA Sequencing:

    Article Title: Engineering a compact CgCas12n platform for efficient genome and base editing in mammalian cells.
    Article Snippet: .. mRNA extraction, real-time quantitative PCR, and RNA-seq Total RNA was extracted from ~1 × 106 sorted cells using TRIzol reagent (Vazyme, #R401-01) and reverse-transcribed using the PrimeScript RT reagent Kit (TaKaRa, #RR037A). .. Quantitative PCR (qPCR) was performed using SYBR Green Mix (ABclonal, #RK21203) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    Reverse Transcription:

    Article Title: Engineering a compact CgCas12n platform for efficient genome and base editing in mammalian cells.
    Article Snippet: .. mRNA extraction, real-time quantitative PCR, and RNA-seq Total RNA was extracted from ~1 × 106 sorted cells using TRIzol reagent (Vazyme, #R401-01) and reverse-transcribed using the PrimeScript RT reagent Kit (TaKaRa, #RR037A). .. Quantitative PCR (qPCR) was performed using SYBR Green Mix (ABclonal, #RK21203) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    Article Title: PagCOBL5 Integrates Transcriptional and Spatial Cues to Confer G-Layer Deposition in Poplar.
    Article Snippet: Angiosperm trees reorient their stems by producing tension wood (TW), a specialised xylem that generates contractile force.. TW fibres develop a gelatinous (G) layer rich in cellulose, yet the molecular mechanisms governing G‐layer formation remain poorly understood.. COBRA‐like genes encode glycosylphosphatidylinositol (GPI)‐anchored proteins known to regulate cellulose deposition.

    Article Title: Dietary Silphium perfoliatum L. Flavonoid extract effects on carcass traits, serum biochemistry, breast meat quality, and myogenic gene expression in broiler chickens.
    Article Snippet: .. RNA concentration and purity were determined, and 1.0 μg of total RNA was reverse-transcribed using PrimeScript RT reagents (Takara Bio Inc., Kusatsu, Shiga, Japan). .. Reverse transcription was performed at 37 ◦C for 15 min followed by 85 ◦C for 5 s. Quantitative real-time PCR was performed using a LightCycler 480 system (Roche Diagnostics GmbH, Mannheim, Germany) with SYBR Green detection.

    Article Title: Exploring the multigenerational effects of Lippia adoensis (Lamiales: Verbenaceae) essential oil and Dichlorvos on Callosobruchus maculatus (Coleoptera: Bruchidae) life-history traits and gene expression
    Article Snippet: .. For first-strand complementary DNA (cDNA) synthesis, 1 μg of total RNA from each sample was reverse-transcribed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio, Japan) according to the manufacturer’s instructions. ..

    Article Title: ROS function during the early phase of vascular regeneration in Arabidopsis.
    Article Snippet: qRT-PCR Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen). .. Total RNA (350 or 1000 ng) was reverse transcribed using the PrimeScript TM RT Reagent Kit with gDNA Eraser (Perfect Real Time) (TAKARA). .. qRT-PCR was performed on a 7500 Real-Time PCR System (Applied Biosystems) using THUNDERBIRD TM Next SYBR TM qPCR Mix (TOYOBO).

    Article Title: CircRNA Profiling Identifies hsa_circRNA_003201 Associated with a Predicted NLRX1-Related Regulatory Network in an Acne-Associated Inflammatory Sebocyte Model
    Article Snippet: .. For cDNA synthesis, miRNAs were reverse transcribed using the miRNA cDNA First Strand Synthesis Kit (Accurate Biology) with stem-loop primers, whereas circRNAs and mRNAs were reverse transcribed using the PrimeScript RT Reagent Kit (Takara) according to the manufacturer’s instructions. ..

    Article Title: The transcriptional regulator ArcR represses virulence and γ-hemolysin expression in Staphylococcus aureus USA300 LAC.
    Article Snippet: Cells were collected by centrifugation at 12000 × g for 2 min. Total RNA was extracted using RNAiso Plus reagent (AKF0727A; TaKaRa) following the manufacturer's instructions. .. One microgram of total RNA was reverse-transcribed using the PrimeScript RT Reagent Kit (RR047A; TaKaRa) to generate cDNA. .. Quantitative real-time PCR (RTqPCR) was performed using the CFX96 real-time system (Bio-Rad) with the RealStar Fast SYBR qPCR Mix (20AE03; GenStar).

    Article Title: Microglial PGRN downregulation contributes to phagocytosis-mediated retinal microvascular endothelial cell damage in early experimental diabetes
    Article Snippet: Total RNA was extracted from each retina and cultured BV-2 cells using TRIzol reagent (Cat.15596018CN, Invitrogen) and the TaKaRa MiniBEST Universal RNA Extraction Kit (Cat.9767, Takara, Kusatsu, Japan), respectively. .. RNA was reverse transcribed using the PrimeScript RT reagent Kit with gDNA Eraser (Cat.RR047A, Takara). .. The cDNA was amplified using the TB Green Premix Ex Taq II (#RR820A, Takara, Japan).

    Isolation:

    Article Title: PagCOBL5 Integrates Transcriptional and Spatial Cues to Confer G-Layer Deposition in Poplar.
    Article Snippet: Angiosperm trees reorient their stems by producing tension wood (TW), a specialised xylem that generates contractile force.. TW fibres develop a gelatinous (G) layer rich in cellulose, yet the molecular mechanisms governing G‐layer formation remain poorly understood.. COBRA‐like genes encode glycosylphosphatidylinositol (GPI)‐anchored proteins known to regulate cellulose deposition.

    RNA Extraction:

    Article Title: PagCOBL5 Integrates Transcriptional and Spatial Cues to Confer G-Layer Deposition in Poplar.
    Article Snippet: Angiosperm trees reorient their stems by producing tension wood (TW), a specialised xylem that generates contractile force.. TW fibres develop a gelatinous (G) layer rich in cellulose, yet the molecular mechanisms governing G‐layer formation remain poorly understood.. COBRA‐like genes encode glycosylphosphatidylinositol (GPI)‐anchored proteins known to regulate cellulose deposition.

    Concentration Assay:

    Article Title: Dietary Silphium perfoliatum L. Flavonoid extract effects on carcass traits, serum biochemistry, breast meat quality, and myogenic gene expression in broiler chickens.
    Article Snippet: .. RNA concentration and purity were determined, and 1.0 μg of total RNA was reverse-transcribed using PrimeScript RT reagents (Takara Bio Inc., Kusatsu, Shiga, Japan). .. Reverse transcription was performed at 37 ◦C for 15 min followed by 85 ◦C for 5 s. Quantitative real-time PCR was performed using a LightCycler 480 system (Roche Diagnostics GmbH, Mannheim, Germany) with SYBR Green detection.

    cDNA Synthesis:

    Article Title: Exploring the multigenerational effects of Lippia adoensis (Lamiales: Verbenaceae) essential oil and Dichlorvos on Callosobruchus maculatus (Coleoptera: Bruchidae) life-history traits and gene expression
    Article Snippet: .. For first-strand complementary DNA (cDNA) synthesis, 1 μg of total RNA from each sample was reverse-transcribed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio, Japan) according to the manufacturer’s instructions. ..

    Article Title: CircRNA Profiling Identifies hsa_circRNA_003201 Associated with a Predicted NLRX1-Related Regulatory Network in an Acne-Associated Inflammatory Sebocyte Model
    Article Snippet: .. For cDNA synthesis, miRNAs were reverse transcribed using the miRNA cDNA First Strand Synthesis Kit (Accurate Biology) with stem-loop primers, whereas circRNAs and mRNAs were reverse transcribed using the PrimeScript RT Reagent Kit (Takara) according to the manufacturer’s instructions. ..



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