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reverse transcribed intocdnawith thehigh capacity cdnareverse transcription kit  (TaKaRa)


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    TaKaRa reverse transcribed intocdnawith thehigh capacity cdnareverse transcription kit
    Reverse Transcribed Intocdnawith Thehigh Capacity Cdnareverse Transcription Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14793 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Reverse Transcription:

    Article Title: Regulatory effects of soy isoflavones on ovarian development in female Chinese mitten crabs ( Eriocheir sinensis )
    Article Snippet: The total RNA quality and concentration were estimated via 1% agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, USA). .. Only samples exhibited clear electrophoretic bands and meeting the 1.8 to 2.0 standard were reverse transcribed via PrimeScript RT Reagent Kit (RR037B, Takara Biotechnology Co., Ltd., Dalian, Liaoning, China). .. The PCR system and procedure were described in the prior study , and RT–qPCR was conducted via a CFX96 Real-Time PCR system (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

    Article Title: Upregulation of miR-589-3p Contributes to Lung Adenocarcinoma Progression Through Inhibition of WWC2.
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. For miRNA detection, reverse transcription was performed using a miRNA First Strand cDNA Synthesis Kit (Takara, Shiga, Japan), while mRNA was reverse-transcribed using a PrimeScript RT reagent Kit (Takara). qRT-PCR was conducted using SYBR Green Master Mix (Takara) on a QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: A dual-responsive CO-releasing nanogel ameliorates retinal ischemia–reperfusion injury by restoring mitochondrial homeostasis and attenuating cGAS-STING pathway activation
    Article Snippet: Total RNA was extracted from R28 cells or mouse retinal tissues via TRIzol reagent (Invitrogen), and the RNA concentration and purity were measured via a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). .. For cDNA synthesis, 1 μg of total RNA was reverse transcribed via the PrimeScriptTM RT Reagent Kit (Takara, Cat# RR037A) according to the manufacturer's protocol. .. Quantitative real-time PCR (qPCR) was performed via SYBR Green Master Mix (Thermo Fisher Scientific, Cat# A25742) on a CFX96 Real-Time PCR Detection System (Bio-Rad).

    Article Title: The inhibitors of miR-224-5p, miR-339-5p, and miR-1198-5p improve auditory function by promoting the expression of neuritin in hearing loss mice
    Article Snippet: To detect miRNAs expression, total RNA was extracted from the organ of Corti using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA). .. RNA samples were reverse-transcribed using the PrimeScriptTM RT Kit (Takara Bio, Shiga, Japan). .. All primers used in this study were synthesized by RiboBio Co., Ltd. (Guangzhou, China, ).

    Article Title: Mechanism of Scutellaria baicalensis extracellular vesicles in attenuating Mycoplasma gallisepticum -induced inflammation via TRPC1 - STIM1/ORAI1 channel inhibition
    Article Snippet: Samples were observed using an upright microscope (NIKON ECLIPSE C1), and images were processed (coloring and merging) with Slide Viewer software. .. Total RNA was isolated from HD-11 cells and tissues using Trizol reagent (TaKaRa, Dalian, China) and reverse transcribed with the PrimeScriptTM Fast RT Reagent Kit with gDNA Eraser (TaKaRa). ..

    Article Title: Butyrate-producing gut bacteria restrain PBAT microplastic-triggered brain microglial lipotoxicity via a microbiota-butyrate-mTORC1-ISR relay along the gut-brain axis.
    Article Snippet: .. Quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted with TRIzol reagent (Ambion, Austin, TX, USA) and reverse-transcribed using PrimeScriptTM RT kit (TaKaRa, Shiga, Japan). qRT-PCR was performed using primers listed in Supplementary Table S3. ..

    Article Title: Human 3D liver spheroids support productive infection of a novel tick-borne phenuivirus
    Article Snippet: Albumin (ALB) and urea were quantified with the Human Serum Albumin ELISA Kit (4 A BIOTECH, China) and Urea Assay Kit E2020 (Applygen Technologies, China), respectively [ , ]. .. Total RNA was extracted from spheroids (72 hpi) using the RNA-easy Isolation Reagent (Vazyme, China) and reverse transcribed (PrimeScriptTM RT reagent Kit with gDNA Eraser,TaKaRa, Japan). qPCR was conducted with TB Green® Premix Ex TaqTM II (TaKaRa, Japan) under the following conditions: 95 °C for 30 s; 40 cycles of 95 °C for 5 s and 60 °C for 30 s. Primers are listed in Table S1. ..

    cDNA Synthesis:

    Article Title: Upregulation of miR-589-3p Contributes to Lung Adenocarcinoma Progression Through Inhibition of WWC2.
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. For miRNA detection, reverse transcription was performed using a miRNA First Strand cDNA Synthesis Kit (Takara, Shiga, Japan), while mRNA was reverse-transcribed using a PrimeScript RT reagent Kit (Takara). qRT-PCR was conducted using SYBR Green Master Mix (Takara) on a QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: A dual-responsive CO-releasing nanogel ameliorates retinal ischemia–reperfusion injury by restoring mitochondrial homeostasis and attenuating cGAS-STING pathway activation
    Article Snippet: Total RNA was extracted from R28 cells or mouse retinal tissues via TRIzol reagent (Invitrogen), and the RNA concentration and purity were measured via a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). .. For cDNA synthesis, 1 μg of total RNA was reverse transcribed via the PrimeScriptTM RT Reagent Kit (Takara, Cat# RR037A) according to the manufacturer's protocol. .. Quantitative real-time PCR (qPCR) was performed via SYBR Green Master Mix (Thermo Fisher Scientific, Cat# A25742) on a CFX96 Real-Time PCR Detection System (Bio-Rad).

    Quantitative RT-PCR:

    Article Title: Upregulation of miR-589-3p Contributes to Lung Adenocarcinoma Progression Through Inhibition of WWC2.
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. For miRNA detection, reverse transcription was performed using a miRNA First Strand cDNA Synthesis Kit (Takara, Shiga, Japan), while mRNA was reverse-transcribed using a PrimeScript RT reagent Kit (Takara). qRT-PCR was conducted using SYBR Green Master Mix (Takara) on a QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: Butyrate-producing gut bacteria restrain PBAT microplastic-triggered brain microglial lipotoxicity via a microbiota-butyrate-mTORC1-ISR relay along the gut-brain axis.
    Article Snippet: .. Quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted with TRIzol reagent (Ambion, Austin, TX, USA) and reverse-transcribed using PrimeScriptTM RT kit (TaKaRa, Shiga, Japan). qRT-PCR was performed using primers listed in Supplementary Table S3. ..

    SYBR Green Assay:

    Article Title: Upregulation of miR-589-3p Contributes to Lung Adenocarcinoma Progression Through Inhibition of WWC2.
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. For miRNA detection, reverse transcription was performed using a miRNA First Strand cDNA Synthesis Kit (Takara, Shiga, Japan), while mRNA was reverse-transcribed using a PrimeScript RT reagent Kit (Takara). qRT-PCR was conducted using SYBR Green Master Mix (Takara) on a QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Upregulation of miR-589-3p Contributes to Lung Adenocarcinoma Progression Through Inhibition of WWC2.
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. For miRNA detection, reverse transcription was performed using a miRNA First Strand cDNA Synthesis Kit (Takara, Shiga, Japan), while mRNA was reverse-transcribed using a PrimeScript RT reagent Kit (Takara). qRT-PCR was conducted using SYBR Green Master Mix (Takara) on a QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: Butyrate-producing gut bacteria restrain PBAT microplastic-triggered brain microglial lipotoxicity via a microbiota-butyrate-mTORC1-ISR relay along the gut-brain axis.
    Article Snippet: .. Quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted with TRIzol reagent (Ambion, Austin, TX, USA) and reverse-transcribed using PrimeScriptTM RT kit (TaKaRa, Shiga, Japan). qRT-PCR was performed using primers listed in Supplementary Table S3. ..

    Article Title: Human 3D liver spheroids support productive infection of a novel tick-borne phenuivirus
    Article Snippet: Albumin (ALB) and urea were quantified with the Human Serum Albumin ELISA Kit (4 A BIOTECH, China) and Urea Assay Kit E2020 (Applygen Technologies, China), respectively [ , ]. .. Total RNA was extracted from spheroids (72 hpi) using the RNA-easy Isolation Reagent (Vazyme, China) and reverse transcribed (PrimeScriptTM RT reagent Kit with gDNA Eraser,TaKaRa, Japan). qPCR was conducted with TB Green® Premix Ex TaqTM II (TaKaRa, Japan) under the following conditions: 95 °C for 30 s; 40 cycles of 95 °C for 5 s and 60 °C for 30 s. Primers are listed in Table S1. ..

    Isolation:

    Article Title: Mechanism of Scutellaria baicalensis extracellular vesicles in attenuating Mycoplasma gallisepticum -induced inflammation via TRPC1 - STIM1/ORAI1 channel inhibition
    Article Snippet: Samples were observed using an upright microscope (NIKON ECLIPSE C1), and images were processed (coloring and merging) with Slide Viewer software. .. Total RNA was isolated from HD-11 cells and tissues using Trizol reagent (TaKaRa, Dalian, China) and reverse transcribed with the PrimeScriptTM Fast RT Reagent Kit with gDNA Eraser (TaKaRa). ..

    Article Title: Human 3D liver spheroids support productive infection of a novel tick-borne phenuivirus
    Article Snippet: Albumin (ALB) and urea were quantified with the Human Serum Albumin ELISA Kit (4 A BIOTECH, China) and Urea Assay Kit E2020 (Applygen Technologies, China), respectively [ , ]. .. Total RNA was extracted from spheroids (72 hpi) using the RNA-easy Isolation Reagent (Vazyme, China) and reverse transcribed (PrimeScriptTM RT reagent Kit with gDNA Eraser,TaKaRa, Japan). qPCR was conducted with TB Green® Premix Ex TaqTM II (TaKaRa, Japan) under the following conditions: 95 °C for 30 s; 40 cycles of 95 °C for 5 s and 60 °C for 30 s. Primers are listed in Table S1. ..



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